Journal of Natural Products Discovery
type: channel
description: JOURNAL OF NATURAL PRODUCTS DISCOVERY (JNPD) is devoted to the publication of original articles The Journal publishes papers describing significant novelty in the analysis, isolation of metabolites, biological and pharmacological properties of natural products (including whole plants, marine organisms, terrestrial animals, fungi, bacteria and viruses, their cells, tissues and organs. The derived extracts and isolates should have characteristics or activities relevant for either basic biological sciences (botany, ecology, biochemistry, pharmacology, etc.) or use in the food, agrochemical, pharmaceutical, cosmetic and related industries. All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products are also within the scope of the journal. Papers dealing with novel methods relating to areas such as data handling / data mining in plant sciences will also be welcomed.
publisher: Liverpool John Moores University
language: en
publication name: Journal of Natural Products Discovery
issn: 2755-1997
copyright: This journal provides immediate open access to its content with no submission or publications fees. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a LicenceCreative Commons Attribution License that allows others to read, download, copy, distribute, print, search, or link to the full text of works in this journal. It also allows others to remix, adapt and build upon the work, as long as credit is given to the author(s).
items: Formulation and Characterization of a Transfersome-based Oral Care Gel: Development and Delivery of Encapsulated Lidocaine
items: Pharmacological and therapeutic applications of scorpion venom peptides: A Review
items: 6th annual CNPD Conference 2025
items: Sustained Release of Linalool from DLP 3D-Printed Films for Topical Antimicrobial Applications
items: Three experimental animal parasite models for assessing anthelmintic efficacy and immunopotentiating properties of natural products: protocols and applications
items: Exploring microalgal lipids as anti-virulence agents targeting MDR Vibrio cholerae infection: A Step Toward Developing Herbal Oral Rehydration Salt (ORS) Formulations
items: 3D Printing of Multilayered Hydrogel containing Hyaluronic acid and Linalool
items: Anti- inflammatory response of Zanthoxylum myriacanthum Wall. ex Hook.f. fruit extract
items: Assessment of Aerides odorata's Antimicrobial, Cytotoxic, Thrombolytic, and Antiarthritic Properties: A Comparative In Vitro Analysis of Its Different Parts
items: The Proceedings of the 5th CNPD Conference
items: A systematic review on the detection of carbapenemase-producing Enterobacteriaceae
items: FORMULATION OF AN ANTIOXIDANT HYDROGEL FACE MASK USING AN ORGANIC, SUSTAINABLY SOURCED, STANDARDISED WATER EXTRACT OF YERBA MATE (ILEX PARAGUARIENSIS) AS ACTIVE INGREDIENT.
items: Antimicrobial Activities, phytochemical composition and thin layer chromatography profile of Gossypium hirsutum and Abelmoschus esculentus leaves extracts
items: IN VITRO EVALUATION OF THE ANTIMICROBIAL, TOXIC, ANTI-ARTHRITIC AND THROMBOLYTIC PROPERTIES OF DIFFERENT PARTS OF PLANT ORCHID RHYNCHOSTYLIS RETUSA (L.) BLUME.
items: Soxhlet extraction cycle-dependent diversity in phenolic profile and antioxidant potency of red cabbage (Brassica oleracea var. capitata F. rubra)
items: RESEARCH ACTIVITIES IN THE CENTRE FOR NATURAL PRODUCTS DISCOVERY IN 2023
items: ARTIFICIAL INTELLIGENCE IN NATURAL PRODUCT DISCOVERY: AN INTERVIEW WITH BARD.
items: THE RELEVANCE OF TOPICAL PLANT USAGE IN TRADITIONAL HERBAL MEDICINE: AN INVESTIGATION INTO WEST AFRICAN TRADITIONAL MEDICINAL PLANTS WITH COSMECEUTICAL POTENTIAL IN SOUTHERN GHANA.
items: APOPTOTIC ACTIVITY OF BOSWELLIA CARTERII EXTRACT ON HUMAN NEUTROPHILS.
items: LEGALISING DRUGS OF ABUSE: THE LE CHATELIER THOUGHT EXPERIMENT
items: Quantification of Linalool in 3D Printed Fast-Dissolving Oral Films by A High-Pressure Liquid Chromatography Method
items: How specific is Crispr-Cas9?
items: The Proceedings of the 4th CNPD Conference
items: THE 4th Annual CNPD (Centre for Natural Products Discovery) Conference
items: PHYTOCHEMICAL, ELEMENTAL ANALYSIS AND ANTIFUNGAL ACTIVITY OF ERIGERON FLORIBUNDUS (BILBAO FLEABANE) LEAF EXTRACT AGAINST MALASSEZIA FURFUR
items: APPLICATIONS OF ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPY IN NATURAL PRODUCT RESEARCH
items: https://azljmu07a0101.ljmu-eprints.cdl.cosector.com/JNPD/article/view/732
items: EFFECTS OF IMPORTANT PLANT SPECIES OF TERUEL ON THE PRO-INFLAMMATORY SIGNALLING CASCADE OF HUMAN MONOCYTES.
items: OLAX SUBSCORPIOIDEA OLIV. (OLACACEAE): AN ETHNOMEDICINAL AND PHARMACOLOGICAL REVIEW
items: NUTRITIVE AND MEDICINAL VALUE OF GONGRONEMA LATIFOLIUM

Formulation and Characterization of a Transfersome-based Oral Care Gel: Development and Delivery of Encapsulated Lidocaine
type: item
description: Introduction: This study reports the formulation and development of oral gel-based transfersome nanoformulations prepared via the thin-film method. Lidocaine was incorporated as the active local anaesthetic agent, and different concentrations of excipients were employed to achieve the optimized gel characteristics. Aims: The aim of this research is to design, develop, and optimize a novel oral care gel in combination with lidocaine-entrapped transfersome carriers intended to promote gingival health, and improve the physicochemical and structural characteristics of the formulation for therapeutic applications in oral care. Methods: Hydrogels with lidocaine were manufactured via sequential phase A–D compounding with and without preservative (phenoxyethanol+ethylhexylglycerin or Plantaserve E), then homogenized via magnetic stirring and high-shear mixing to achieve optimum uniformity. Transfersomes were produced by thin-film hydration using a rotary evaporator. These gel-based formulations were then characterized for their rheological properties, texture analysis, and Fourier transform infrared spectroscopy. Lidocaine entrapment efficiency was quantified using the HPLC technique and antimicrobial activity was tested against Candida albicans. Results: The optimized gel incorporated transfersome formulation with lidocaine (NF8) demonstrated near-physiological pH (~6.5) and spreadability of 3.0 cm (the same as the control product, 3.0 cm). The same gel formulation showed a viscosity of ~75,840 cP and ~28,200 cP at 2.5 and 10 rpm. The texture analyzer showed a firmness of 122.3 ± 9.1 g and a shear work of 61.7 ± 9.1 g/sec. Entrapment efficiency of lidocaine in transfersome nanoformulation was found to be 71%, whereas the particle size and zeta potential were found to be 268 nm and -3 mV. Whereas non-sonicated dispersions showed larger transfersome particle size (453 nm) and wide polydispersity (PDI of 0.81). Conclusion: These findings showed that transfersome-based gel has…
subject: Original Articles
  topic: section
subject: Transfersome
  topic: keywords
subject: Thin-film
  topic: keywords
subject: Oral gel
  topic: keywords
subject: Lidocaine
  topic: keywords
creator: Iftikhar Khan
creator: Chahinez Houacine
creator: Mandana Shahreza
rights: Copyright (c) 2025 Iftikhar Khan, Chahinez Houacine, Mandana Javeri Shahreza https://creativecommons.org/licenses/by/4.0
date: 2025-11-06
publication date: 2025-11-06
doi: 10.24377/jnpd.article3407

Pharmacological and therapeutic applications of scorpion venom peptides: A Review
type: item
description: Introduction: In recent years, scorpion venom and its peptide compounds have attracted growing scientific interest. A remarkable biological diversity and strong therapeutic potential characterize these compounds. Aims: to provide a comprehensive review of the therapeutic properties of scorpion venoms. Methods: This analysis is based on a collection of 53 studies published between 2020 and 2025, all of which have strict inclusion criteria and are drawn from reliable sources, including specialized platforms such as PubMed, Web of Science, ScienceDirect, Scopus, and Google Scholar. The studies reviewed focus on the pharmacological effects of scorpion venom and chemical components. Results: The collected data highlight the increased interest in scorpion-derived peptides as pharmacological tools in biomedical research, attributed to their diverse biological actions, including anticancer, antimicrobial, antiviral, antiparasitic, analgesic, antioxidant, immunomodulatory, and neuroprotective. Among the described mechanisms of action are the induction of apoptosis, disruption of cell membranes, modulation of intracellular signalling pathways, and inhibition of ion channels. Most studies on the subject demonstrate significant efficacy, with low toxicity observed in both in vitro cell cultures and in vivo murine models. Conclusions: This review confirms the promising potential of scorpion venom peptides for developing novel therapeutic and biotechnological applications. However, it also emphasizes the necessity of further clinical research and standardized experimental protocols to fully harness their benefits.
subject: Review Articles
  topic: section
subject: Scorpion venom, Peptides, Therapeutic potential, Pharmacological activities, Review
  topic: keywords
creator: Siham Namiq
creator: Btissam BOUIMEJA
creator: Elmourid Abdessamad
creator: Ahmed KHADRA
creator: Oulaid TOULOUN
rights: Copyright (c) 2025 Siham Namiq, Dr. Boumeja Btissam , Dr Elmourid Abdessamad, Pr. Khadra ahmed, Pr. Touloun Oulaid https://creativecommons.org/licenses/by/4.0
date: 2025-11-27
publication date: 2025-11-27
doi: 10.24377/jnpd.article3402

6th annual CNPD Conference 2025
type: item
description: 6th annual CNPD Conference 2025
subject: Conference Abstracts
  topic: section
creator: Touraj Ehtezazi
rights: Copyright (c) 2025 Touraj Ehtezazi https://creativecommons.org/licenses/by/4.0
date: 2025-10-08
publication date: 2025-10-08
doi: 10.24377/jnpd.article3372

Sustained Release of Linalool from DLP 3D-Printed Films for Topical Antimicrobial Applications
type: item
description: Background Linalool is a naturally occurring antimicrobial agent with potential applications in dermatological treatments. Achieving sustained and controlled release of such volatile compounds remains a challenge in topical delivery systems. Digital Light Processing (DLP) 3D printing offers precise control over formulation parameters, enabling the fabrication of customised polymeric films with tailored release properties. Aims This study aimed to investigate the sustained-release characteristics of linalool from DLP-printed polymeric films, examining the influence of formulation and printing parameters on release kinetics and therapeutic performance. Method Photocurable resins were loaded with linalool at varying concentrations and processed into films using DLP 3D printing under different conditions, including variations in resin type and projector power. Linalool release was quantified using High-Performance Liquid Chromatography (HPLC). Structural and thermal properties were characterised via Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC). Antifungal activity was assessed to determine therapeutic applicability. Results Resin composition and linalool concentration significantly influenced release kinetics, with higher loading levels resulting in increased release rates. Projector power variation did not produce notable changes in the release behaviour. Films containing 20% w/w linalool exhibited strong activity against Staphylococcus aureus demonstrating both efficacy and sustained-release properties. Conclusion DLP 3D printing enables the fabrication of customised polymeric films capable of sustained linalool release, with performance through formulation adjustments. These findings suggest a promising approach for the development of topical antimicrobial delivery systems, particularly for dermatological conditions such as acne.
subject: Original Articles
  topic: section
subject: 3D printing; Hydrogel, Skincare; Linalool; Personalised
  topic: keywords
subject: DLP
  topic: keywords
creator: Touraj Ehtezazi
creator: Satyajit Sarker
creator: Taketo Yagi
rights: Copyright (c) 2025 Touraj Ehtezazi, Satyajit Sarker, Taketo Yagi https://creativecommons.org/licenses/by/4.0
date: 2025-11-06
publication date: 2025-11-06
doi: 10.24377/jnpd.article3355

Three experimental animal parasite models for assessing anthelmintic efficacy and immunopotentiating properties of natural products: protocols and applications
type: item
description: <a href="Introduction: Helminthic infections, including soil-transmitted helminths (STHs) and intestinal tapeworms, pose a major global health challenge, classified as neglected tropical diseases (NTDs). Objective: Current control efforts are hampered by expensive treatments, emerging drug resistance, and high reinfection rates. This necessitates the urgent discovery of new anthelmintic drugs and immunomodulatory compounds. Natural products are a promising source for these new therapies. Materials and methods: This paper introduces three experimental animal parasite models specifically designed to evaluate the effectiveness and immune-boosting properties of natural products, namely Hymenolepis diminuta (tapeworm) in mice, Ascaridia galli (nematode) in chickens, and Raillietina cesticillus (tapeworm) in chickens. Rationale: These models provide controlled environments for assessing potential therapeutic compounds and offer systematic methodologies for identifying effective and immunomodulatory natural products. Conclusions: A significant challenge in treating helminth infections is targeting larval stages embedded in host tissues, such as those seen with Hymenolepis nana in humans and hypobiotic trichostrongylid nematode larvae in ruminants. Therefore, demonstrating a natural product's effectiveness against A. galli larvae embedded in the intestinal mucosa is particularly important. Such efficacy would indicate that the product possesses valuable physicochemical and pharmacological properties, paving the way for developing novel treatments against hidden, tissue-dwelling parasites in both humans and animals.">Introduction: Helminthic infections, including soil-transmitted helminths (STHs) and intestinal tapeworms, pose a major global health challenge, classified as neglected tropical diseases (NTDs). Objective: Current control efforts are hampered by expensive treatments, emerging drug resistance, and high reinfection rates. This necessitates the urgent discovery of …
subject: Opinion papers
  topic: section
subject: : INTESTINAL HELMINTHS; ANIMAL MODELS; NATURAL PRODUCTS; ANTHELMINTIC EFFICACY; IMMUNOPOTENTIATION
  topic: keywords
creator: Elsayed Elowni
rights: Copyright (c) 2025 Elsayed Elowni https://creativecommons.org/licenses/by/4.0
date: 2025-11-06
publication date: 2025-11-06
doi: 10.24377/jnpd.article3245

Exploring microalgal lipids as anti-virulence agents targeting MDR Vibrio cholerae infection: A Step Toward Developing Herbal Oral Rehydration Salt (ORS) Formulations
type: item
description: Introduction:Cholera, a life-threatening diarrheal disease caused by Vibrio cholerae, remains a major global health issue, especially in tropical regions. Increasing antibiotic resistance in V. cholerae strains poses a significant threat to effective treatment. Novel antivirulence strategies that do not promote resistance are urgently needed. Objectives: This study investigates the potential of lipid extracts and crude biomass derived from Chlorella variabilis (CV) and Chlorococcum sp. (CCM) microalgae to inhibit cholera toxin (CT) production by a multidrug-resistant V. cholerae strain (SRK-19), without affecting bacterial viability. Materials and Methods: Lipid extracts and crude freeze-dried biomass from CV and CCM were tested against V. cholerae SRK-19 in vitro for their effects on CT production (measured via ELISA) and bacterial viability. For in vivo validation, a rabbit ileal loop assay was performed, and fluid accumulation (FA ratio), colony-forming units (CFU), and CT levels were measured. Results: Both lipid extracts and crude biomass significantly reduced CT production in vitro in a dose-dependent manner, with up to 97.9% inhibition observed using CCM lipid extract at 150 μg/ml. Crude extracts achieved comparable inhibition, with CV and CCM biomass reducing CT levels by 93% and 97%, respectively, at 1 mg/ml. In vivo studies confirmed reduced FA ratios and CT levels in treated ileal loops, without affecting bacterial growth (CFU counts remained unchanged), indicating that bacterial viability was not compromised. Conclusion: Crude and lipid extracts from CV and CCM demonstrate potent antivirulence activity against V. cholerae by inhibiting CT production without promoting antimicrobial resistance. These findings support the development of a microalgae-based oral rehydration formulation as a promising alternative to conventional antibiotic therapy for cholera.
subject: Original Articles
  topic: section
subject: Cholera toxin; Microalgae; Lipid extracts; in-vivo study; anti-virulence
  topic: keywords
creator: Soumya Haldar
creator: Sweta Jaiswal
creator: Nikita Vadadoriya
creator: Nasir Abdul
creator: Ramalingam Dineshkumar
creator: Neeraj Khatri
creator: Sachin Raut
creator: Saumya Ray Chaudhuri
creator: Shruti Chatterjee
rights: Copyright (c) 2025 Soumya Haldar, Sweta Jaiswal, Nikita , Nasir Abdul, Ramalingam Dineshkumar, Neeraj Khatri, Sachin Raut, Saumya Ray Chaudhuri, Shruti Chatterjee https://creativecommons.org/licenses/by/4.0
date: 2025-11-06
publication date: 2025-11-06
doi: 10.24377/jnpd.article3244

3D Printing of Multilayered Hydrogel containing Hyaluronic acid and Linalool
type: item
description: This study presents the 3D printing and the development of multilayered hydrogel formulations containing hyaluronic acid (HA) and linalool (LN) for potential use as a personalised moisturising and anti-acne product. Various shapes were printed via 3D printing for personalised application; however, material characterisation was conducted primarily on the first layer. The average film weight was 2.11 ± 0.20 g, with optimal storage conditions identified as placement on a covered plate at 25 °C. Films containing 10% LN demonstrated a higher linalool release profile (maximum 22.66% of the nominal dose) compared to the 5% LN films, correlating with stronger antibacterial activity against Staphylococcus aureus. FT-IR analysis revealed the absence of C=C and C–H peaks in blank and 5% LN films due to water interference, while 10% LN films displayed characteristic LN peaks. Texture analysis showed that the 5% LN film exhibited greater tensile resistance, with the highest force recorded at 27.500 g. LN played an important role in the transformation of hydrogel ink to a liquid-like state under high strain. In conclusion, these findings highlight the potential of 3D-printed HA–LN hydrogels for customisable skincare applications with dual functionality in moisturising and antimicrobial effects.
subject: Original Articles
  topic: section
subject: 3D printing; Hydrogel, Skincare; Linalool; Personalised
  topic: keywords
creator: Punnatron Kongsithiseth
creator: Satyajit Sarker
creator: Touraj Ehtezazi
rights: Copyright (c) 2025 Punnatron Kongsithiseth, Satyajit Sarker, Touraj Ehtezazi https://creativecommons.org/licenses/by/4.0
date: 2025-10-08
publication date: 2025-10-08
doi: 10.24377/jnpd.article3240

Anti- inflammatory response of Zanthoxylum myriacanthum Wall. ex Hook.f. fruit extract
type: item
description: Background Zanthoxylum myriacanthum, known as Ma-khwaen, is widely used in Thai folk medicine and as a culinary spice. The whole fruits, including pericarp and seeds, extracted with 95% ethanol has anti-inflammatory potential, but the mechanisms of action need to be validated. Aims To investigate the anti-inflammatory effect of fruit ethanolic extract. Methods The extract, prepared via 95% ethanolic maceration, underwent HPLC analysis for quality control using L-asarinin as a standard. Its anti-inflammatory effects were evaluated using the cyclooxygenase inhibitor assay and RAW 264.7 macrophage cells through MTT cytotoxicity, COX inhibition, and RT-PCR assays, measuring inflammatory gene expression (COX-1, COX-2, IL-1β, IL-6, TNF-α). Statistical analysis (One-way ANOVA) assessed the significance of findings. Results ZL1 extract, obtained with a 14.32% yield and containing 0.040 % w/w L-asarinin, demonstrated an IC50 of 102.46 µg/mL on RAW 264.7 cells. Notably, ZL1 inhibited COX-2 activity in a dose-dependent manner, while showing no effect on COX-1. Gene expression analysis in LPS-inflamed RAW 264.7 cells further revealed that ZL1 down-regulated COX-2, IL-1β, IL-6, and TNF-α at concentrations as low as 12.5 µg/mL, indicating its selective anti-inflammatory potential. Conclusion Zanthoxylum myriacanthum ethanolic extract (ZL1) selectively inhibited COX-2. It significantly suppressed inflammatory genes (COX-2, IL-1β, IL-6, TNF-α) in LPS-induced RAW264.7 cells at 12.50, 25 and 50 µg/mL, demonstrating its potent anti-inflammatory effects.
subject: Original Articles
  topic: section
subject: Anti- inflammatory
  topic: keywords
subject: Zanthoxylum myriacanthum
  topic: keywords
subject: COX inhibition
  topic: keywords
creator: Sarayut Radapong
creator: Tiyanee Sahad
creator: Nathaphat Harnkit
creator: Praw Suppajariyawat
creator: Detmontree Wachisunthon
creator: Satyajit D. Sarker
rights: Copyright (c) 2025 Sarayut Radapong, Tiyanee Sahad, Nathaphat Harnkit, Praw Suppajariyawat, Detmontree Wachisunthon, Satyajit D. Sarker https://creativecommons.org/licenses/by/4.0
date: 2025-10-08
publication date: 2025-10-08
doi: 10.24377/jnpd.article3231

Assessment of Aerides odorata's Antimicrobial, Cytotoxic, Thrombolytic, and Antiarthritic Properties: A Comparative In Vitro Analysis of Its Different Parts
type: item
description: Historically, thrombosis, arthritis, cancer, wounds, and infections have all been treated with various herbal preparations. The aim of this work was to evaluate the antibacterial, thrombolytic, cytotoxic, and protein denaturation activities of ethanolic extracts from various Aerides odorata (AO) sections in vitro. Fourteen distinct microorganisms were used in the antimicrobial study; for orchid samples, ciprofloxacin was the most effective agent. AO/Leaf was the only one to exhibit antimicrobial potential; AO/Stem and AO/Root did not. Based on their LC50 values, we evaluated the cytotoxicity of plant extracts. AO/Root extract had the greatest cytotoxic activity when compared to AO/Leaf and AO/Stem extracts. Two incubation times (1.5 hours and 24 hours) and two concentrations (one thousand and one hundred ppm) were used to assess the thrombolytic activity of the extracts. The results obtained with regular streptokinase were then compared with the former. Compared to AO/Leaf and AO/Stem extract, it was discovered that AO/Root extract exhibited more thrombolytic activity. Furthermore, the herbal extract's protein denaturation was investigated at four different concentrations, and the results were contrasted with those of Diclofenac sodium. In relation to other sections, the maximum protein denaturation values for AO/Stem extract were determined to be 60.07±2.33%, 47.22±1.43%, 35.05±2.07 %, and 23.74±1.66% for 500–62.5 ppm, respectively.
subject: Original Articles
  topic: section
subject: Aerides odorata
  topic: keywords
subject: Traditional Herbal Medicine
  topic: keywords
subject: Antimicrobial Activities
  topic: keywords
subject: Brine Shrimp Lethality Bioassay
  topic: keywords
subject: Anti-arthritic
  topic: keywords
subject: Thrombolytic
  topic: keywords
creator: Md. Saifur Rahaman Saifur
creator: Md. Samsur Rahaman
creator: 3. Shah Md. Marzuk Hasnine
creator: Tanvir Ahmed
creator: Salma Sultana
creator: Md. Abdul Quaiyum Bhuiyan
creator: Jahid Md. Mahbub Islam
creator: Md. Ismail Hossain
creator: Mubarak A Khan
rights: Copyright (c) 2025 Md. Saifur Rahaman Saifur, Md. Samsur Rahaman, 3. Shah Md. Marzuk Hasnine, Tanvir Ahmed, Salma Sultana, Md. Abdul Quaiyum Bhuiyan, Jahid Md. Mahbub Islam, Md. Ismail Hossain, Mubarak A Khan https://creativecommons.org/licenses/by/4.0
date: 2025-10-08
publication date: 2025-10-08
doi: 10.24377/jnpd.article3180

The Proceedings of the 5th CNPD Conference
type: item
description: This online conference marks the 5th anniversary of the foundation of the CNPD, which conducts externally funded and impactful natural products research with the aim of discovering new high-value natural products that will contribute to tackling current and future societal and global challenges in health and well-being, socio-economic growth, natural conservation, and environmental sustainability.
subject: Conference Abstracts
  topic: section
subject: Natural Products
  topic: keywords
subject: Conference
  topic: keywords
subject: Drug Discovery
  topic: keywords
creator: Satyajit D. Sarker
creator: Touraj Ehtezazi
creator: Alistair Fielding
creator: Francesca Giuntini
creator: Lutfun Nahar
creator: Ismini Nakouti
creator: Jose M Prieto-Garcia
rights: Copyright (c) 2025 Satyajit D. Sarker, Touraj Ehtezazi, Alistair Fielding, Francesca Giuntini, Lutfun Nahar , Ismini Nakouti; Jose M Prieto-Garcia https://creativecommons.org/licenses/by/4.0
date: 2025-01-06
publication date: 2025-01-06
doi: 10.24377/jnpd.article2791

A systematic review on the detection of carbapenemase-producing Enterobacteriaceae
type: item
description: Introduction: Carbapenemase-producing Enterobacteriaceae (CPE) represents one of the most pressing and critical public health challenges associated with antibiotic resistance. Challenges persist in accurately and promptly identifying CPE despite the existence of diverse carbapenemases and multiple detection methods. Aim: This study investigated diagnostic methods used for the detection of CPEs. Methods: The systematic review and meta-analysis were conducted based on Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines. Electronic databases like Google Scholar, PubMed, Scopus, and Web of Science were used to find relevant articles. In addition, the Joanna Briggs Institute quality appraisal tool was used to assess the quality of the included studies. STATA 14.0 was used for statistical analysis. Heterogeneity was assessed by using Cochran’s Q test and 12 statistics. In addition, publication bias was assessed using a funnel plot and Egger’s test. A random effect model was used to estimate the pooled prevalence. Results: The meta-analysis revealed an overall pooled proportion of 40.53% for phenotypic detection of carbapenemase activity across the 11 studies, with substantial heterogeneity observed. Subgroup analysis highlighted variations in detection proportions based on different methods, with mCIM showing the highest proportion at 58,20%, Carba NP at 27.79%, and MHT at 34,62%. Evaluation of publication bias indicated little impact on the results, maintaining the stability of the meta-analysis outcomes. Conclusion: In conclusion, this systematic review showed a high prevalence of CPE across the studies. This study emphasizes the importance of standardized detection methods, global collaboration, and the integration of advanced techniques for accurate CPE detection.
subject: Review Articles
  topic: section
subject: Enterobacteriaceae, carbapenem, CPE, phenotypic method, genotypic method, Meta-analysis, systematic review.
  topic: keywords
creator: Ismini Nakouti
rights: Copyright (c) 2024 Ismini Nakouti https://creativecommons.org/licenses/by/4.0
date: 2025-03-11
publication date: 2025-03-11
doi: 10.24377/jnpd.article2797

FORMULATION OF AN ANTIOXIDANT HYDROGEL FACE MASK USING AN ORGANIC, SUSTAINABLY SOURCED, STANDARDISED WATER EXTRACT OF YERBA MATE (ILEX PARAGUARIENSIS) AS ACTIVE INGREDIENT.
type: item
description: Background: The use of natural products in cosmetics has become increasingly popular in recent years. One such natural product that has shown promise as a cosmetic ingredient is Ilex paraguariensis, also known as yerba mate. Aim: to formulate an antioxidant hydrogel face mask using an organic, sustainably sourced, standardised water extract of yerba mate (Ilex paraguariensis) as active ingredient. Methods: Organic yerba mate was water-extracted at 40°C and 80°C. Theobromine, theophylline, caffeine, and various phenolic acids were quantified using RP-HPLC-UV analysis. Extracts were then evaluated for antioxidant activity (DPPH assay). A commercially available mask-making machines for home use was used to produce hydrogel masks based on marine collagen and these were analysed for active ingredient distribution (homogeneity). Results: The differences between 40°C and 80°C water extracts of yerba mate. The content in phytochemicals ranged 0.02-1 mg/ml. The extract equivalence in chlorogenic acid (CA) according to the DPPH assay was 0.25 mgCA / mgextract. The hydrogel facial mask was successfully formulated with 0.1 mg/mL of standardised yerba mate extract. The content in phytochemicals within the gel varied most for caffeic acid and theobromine (approx. 20%) but was <10% for theophylline, caffeine, chlorogenic acid, ferulic acid, rutin, quercitrin, and quercetin. Conclusion: Face masks “home” machines can deliver hydrogel face masks based on marine collagen can incorporate yerba mate (Ilex paraguariensis) water extracts as active ingredient without detriment of the consistency, texture and sensory characteristics with relatively homogenous concentrations of the active principles.
subject: Original Articles
  topic: section
subject: Cosmetic Natural Product
  topic: keywords
subject: Antioxidant
  topic: keywords
subject: Polyphenols
  topic: keywords
subject: Xanthine alkaloids
  topic: keywords
creator: Faten Mokhtar
creator: Jose M Prieto-Garcia
rights: Copyright (c) 2024 Faten Mokhtar , Jose M Prieto-Garcia https://creativecommons.org/licenses/by/4.0
date: 2024-12-28
publication date: 2024-12-28
doi: 10.24377/jnpd.article2199

Antimicrobial Activities, phytochemical composition and thin layer chromatography profile of Gossypium hirsutum and Abelmoschus esculentus leaves extracts
type: item
description: Background: This study evaluates the antimicrobial activities, phytochemical composition, and thin layer chromatography (TLC) profile of ethanolic and aqueous leaf extracts of Gossypium hirsutum L. and Abelmoschus esculentus (L.) Moench. Aims: The objective is to investigate the phytochemical constituents and assess the antimicrobial efficacy of these plant extracts against various pathogenic microorganisms. Methods: Leaves were collected, identified, air-dried, and powdered. Ethanolic and aqueous extracts were prepared and subjected to TLC analysis using silica gel plates and a mobile phase of ethanol, methanol, and ethyl acetate (5:3:2). Phytochemical screening was conducted using standard protocols. Antimicrobial activity was evaluated using the agar well diffusion method against pathogens including Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Proteus, Streptococcus pneumoniae and Candida spp. Results: TLC analysis revealed seven compounds in G. hirsutum and six in Abelmoschus esculentus with distinct Rf values. Phytochemical screening identified phenols/tannins, saponins, flavonoids, reducing sugars, terpenoids, and anthraquinones in G. hirsutum, while A. esculentus contained all tested phytochemicals except phytosterol and essential oil. The antimicrobial assay indicated that ethanolic extracts of both plants exhibited significant antibacterial activity, with G. hirsutum showing higher efficacy. Ethanolic extracts of G. hirsutum inhibited S. aureus and S. typhi effectively, while aqueous extracts showed limited activity. Extracts of A. esculentus leaves inhibited all tested organisms except Proteus, with ethanol extracts also ineffective against S. pneumoniae. Conclusion: The study demonstrates that ethanolic extracts of G. hirsutum and A. esculentus possess antimicrobial properties. These findings support the potential use of these plant extracts in developing natural antimicrobial agents.
subject: Original Articles
  topic: section
subject: Antimicrobial
  topic: keywords
subject: Phytochemicals
  topic: keywords
subject: Thin Layer Chromatography
  topic: keywords
subject: Gossypium hirsutum
  topic: keywords
subject: Abelmoschus esculentus
  topic: keywords
creator: Chika Attama
creator: Luka Lawrence
creator: Olanipekun Gabriel Segun
rights: Copyright (c) 2024 Chika Attama, Luka Lawrence, Olanipekun Gabriel Segun https://creativecommons.org/licenses/by/4.0
date: 2024-10-15
publication date: 2024-10-15
doi: 10.24377/jnpd.article2579

IN VITRO EVALUATION OF THE ANTIMICROBIAL, TOXIC, ANTI-ARTHRITIC AND THROMBOLYTIC PROPERTIES OF DIFFERENT PARTS OF PLANT ORCHID RHYNCHOSTYLIS RETUSA (L.) BLUME.
type: item
description: Background: Infection due to microbes, trauma, cancer, arthritis and thrombosis are the most common problems worldwide. Traditionally, a large number of herbal extracts are used for the treatment of those disorders. Aim: Ethanolic extracts from leaves, roots and stems of Rhynchostylis retusa (L.) Blume were phytochemically characterised and then tested for their antibacterial, toxicity, anti-arthritic, and thrombolytic properties in-vitro. Methods and Results: Disk diffusion inhibition tests with a panel of fourteen different microorganisms was used as the antimicrobial assays. Antimicrobial activity of the stem extract found to be higher (10-11 mm, 400 µg/disk) than that of root extract whereas leaf showed no activity with ciprofloxacin (43-46 mm, 5 µg/disc) serving as reference standard. Toxicity of the plant extracts in terms of their LC50 values against the brine shrimp (A. salina) was assessed. The toxic potential of leaf extract was 6.7 μg/ml, which is higher than that of stem and root extract (c.a. 8.5 μg/ml). Thrombolytic properties were tested in vitro using streptokinase as reference (%Clot lysis after 1.5h & 24h was 76.15±1.94 & 92.59±2.35, 30000iu). The leaf extract exhibited higher activity. Leaf extract (1000 ppm) lysed of 33.08±1.24% of the clot and 60.47±2.33 after 1.5 h and 24 h of incubation, respectively. Anti-arthritic effects measured as albumin denaturation using diclofenac sodium as reference (IC50=60.25 ppm) revealed the stem extract as more active. Conclusion: our research will help to provide evidence for some of the traditional uses of this medicinal plant.
subject: Original Articles
  topic: section
subject: Plant Orchid
  topic: keywords
subject: Traditional Herbal Medicine
  topic: keywords
subject: Antimicrobial Activities
  topic: keywords
subject: Brine Shrimp Lethality Bioassay
  topic: keywords
subject: Anti-arthritic
  topic: keywords
subject: Thrombolytic
  topic: keywords
subject: In Vitro Pharmacological Model
  topic: keywords
creator: Md. Saifur Rahaman
creator: Md. Samsur Rahaman
creator: SHAH MD. MARZUK HASNINE
creator: Salma Sultana
creator: Mohammad Shahriar Kabir
creator: Md. Abdul Bari
creator: Md. Ismail Hossain
creator: Jahid Md. Mahbub Islam
creator: Mubarak Ahmad Khan
creator: Md. Abdul Quaiyum Bhuiyan
rights: Copyright (c) 2024 Md. Saifur Rahaman, Md. Samsur Rahaman, SHAH MD. MARZUK HASNINE, Salma Sultana, Mohammad Shahriar Kabir, Md. Abdul Bari, Md. Ismail Hossain, Jahid Md. Mahbub Islam, Mubarak Ahmad Khan, Md. Abdul Quaiyum Bhuiyan https://creativecommons.org/licenses/by/4.0
date: 2024-06-27
publication date: 2024-06-27
doi: 10.24377/jnpd.article2348

Soxhlet extraction cycle-dependent diversity in phenolic profile and antioxidant potency of red cabbage (Brassica oleracea var. capitata F. rubra)
type: item
description: Introduction: The Brassicaceae vegetables are a rich source of secondary metabolites that exhibit several health benefits and protection against numerous degenerative diseases. Objectives: The current study was performed to investigate the effect of Soxhlet extraction cycles on the phenolic profile of red cabbage (Brassica oleracea var. capitata F. rubra) and its biological activities. Materials and Methods: The red cabbage sample was harvested from the research fields of Ayub Agricultural Research Institute Faisalabad, Pakistan. The ethanol extracts were prepared using 1-, 2-, 4-, and 8-cycles Soxhlet extraction technique. The antioxidant potential of red cabbage extracts was estimated by evaluation of total phenolic contents (TPC), total flavonoid contents (TFC), DPPH radical scavenging capacity and reducing power. The identification and quantification of polyphenols were carried out by RP-HPLC. The antibacterial activity of red cabbage extracts was determined against Escherichia coli and Staphylococcus aureus and the antiproliferative activity was carried out by MTT mitochondrial viability assay against the human A549 cancer cell line. Results: Gallic acid, p-hydroxyl benzoic acid, chlorogenic acid, and p-coumaric acid were the major phenolic acids, whereas catechin and quercetin were the major flavonoids detected in the red cabbage extracts. The extraction cycles were found to have significant (p ≤ 0.05) effects on the phenolic profile of the red cabbage extracts. TPC of extracts ranged from 5.22-11.72 mg/g dry matter, measured as gallic acid equivalent, while the TFC ranged from 1.64-5.19 mg/g dry matter; measured as catechin equivalent. The 4-cycles extract of red cabbage exhibited the maximum TPC, TFC, and DPPH free-radical scavenging and reducing activity. Conclusion: The study concluded that the Soxhlet extraction cycles could exert a considerable effect on the yield and polyphenol composition of red cabbage extracts as well as their antioxidant potential. …
subject: Original Articles
  topic: section
subject: Extraction cycles, Brassica oleracea, Gallic acid, p-hydroxyl benzoic acid, DPPH radical, A549 cancer cell line
  topic: keywords
creator: Abdullah ijaz Hussain
creator: Irsa Zafar
creator: Neelam Iftikhar
creator: Lutfun Nahar
creator: Satyajit D Sarker
rights: Copyright (c) 2024 Abdullah ijaz Hussain, Irsa Zafar, Neelam Iftikhar, Lutfun Nahar, Satyajit D Sarker https://creativecommons.org/licenses/by/4.0
date: 2024-06-28
publication date: 2024-06-28
doi: 10.24377/jnpd.article995

RESEARCH ACTIVITIES IN THE CENTRE FOR NATURAL PRODUCTS DISCOVERY IN 2023
type: item
description: In 2023, the Centre for Natural Products Discovery (CNPD) made significant strides in the scientific community through a series of impactful research initiatives. These efforts underscore our commitment to harnessing the world’s natural resources to advance health. The fertile research environment at the CNPD has given rise to various sections, each contributing innovative and practical applications. Our goal here is to provide a concise overview of our research activities for the year. While this summary is not exhaustive, it focuses on the work of our section leaders, which in turn encompasses the contributions of numerous other researchers both within and outside the CNPD.
subject: Editorial
  topic: section
subject: Natural Products
  topic: keywords
creator: Jose M Prieto-Garcia
creator: Alistair Fielding
creator: Fyaz M. D. Ismail
creator: Ismini Nakouti
creator: Touraj Ehtezazi
creator: Pattanathu K.S.M. Rahman
creator: Amos A. Fatokun
creator: Kenneth J. Ritchie
creator: Satyajit D. Sarker
rights: Copyright (c) 2023 Jose M Prieto-Garcia; Alistair Fielding, Fyaz M. D. Ismail, Ismini Nakouti, Touraj Ehtezazi, Pattanathu K.S.M. Rahman, Amos A. Fatokun, Kenneth J. Ritchie, Satyajit D. Sarker https://creativecommons.org/licenses/by/4.0
date: 2023-12-31
publication date: 2023-12-31
doi: 10.24377/jnpd.article2200

ARTIFICIAL INTELLIGENCE IN NATURAL PRODUCT DISCOVERY: AN INTERVIEW WITH BARD.
type: item
subject: Editorial
  topic: section
creator: Jose M Prieto-Garcia
rights: Copyright (c) 2023 Jose M Prieto-Garcia https://creativecommons.org/licenses/by/4.0
date: 2023-12-29
publication date: 2023-12-29
doi: 10.24377/jnpd.article2198

THE RELEVANCE OF TOPICAL PLANT USAGE IN TRADITIONAL HERBAL MEDICINE: AN INVESTIGATION INTO WEST AFRICAN TRADITIONAL MEDICINAL PLANTS WITH COSMECEUTICAL POTENTIAL IN SOUTHERN GHANA.
type: item
description: Introduction: Cosmetopea, a relatively novel and under-reported field in ethnobotany, is concerned with documenting medicinal plants' cosmetic/skin-related uses across countries and cultures. Aim: To identify Western African plants and plant preparations traditionally used for skin conditions and other skin applications and to determine the activity of a selected medicinal plant. Method: A quantitative ethnobotanical survey was conducted within Southern Ghana to investigate knowledge of medicinal plant species and preparations used topically for various skin conditions amongst local and rural communities. Results: Plant use was cited as highest amongst men (76%) and herbal practitioners (60%). In total, the survey revealed 25 medicinal plants distributed amongst 20 plant families with 6 species being most frequently cited overall, which included Senna alata, Ageratum conyzoides, Momordica charantia, Phyllanthus amarus, Alstonia boonei and Ricinis communis. Major skin applications of most plant species reported were for the treatment of skin diseases whilst lesser applications were recorded for cosmetic use. In terms of skin conditions, pimples/rashes was recorded with a high frequency of citations (34.87%). Mixtures were cited as the most common (62%) preparation type whilst raw application was recorded with the lowest usage (3%). A total of 28 medicinal plant recipes were recorded with the leaf plant part being reported with the highest frequency of usage (64%) in preparations. Mechanical extraction techniques including grinding or crushing were frequently cited (46%) whilst solvent extraction namely by water was reported with the lowest use (3%). Conclusion: It was determined that the uses of such plants are relevant amongst local and rural communities and in the traditional herbal industry in Ghana.
subject: Original Articles
  topic: section
subject: Skin conditions
  topic: keywords
subject: Cosmetics
  topic: keywords
subject: Herbal substances
  topic: keywords
subject: traditional medicine
  topic: keywords
subject: Ethnobotany
  topic: keywords
creator: Victoria Ezekwem
rights: Copyright (c) 2023 Victoria Ezekwem https://creativecommons.org/licenses/by/4.0
date: 2023-12-31
publication date: 2023-12-31
doi: 10.24377/jnpd.article2197

APOPTOTIC ACTIVITY OF BOSWELLIA CARTERII EXTRACT ON HUMAN NEUTROPHILS.
type: item
description: Neutrophil activation is required for the initiation of the defence mechanisms which include phagocytosis. Paradoxically, neutrophils also represent one of the main mediators of tissue injury in various human diseases. The resolution of inflammation requires getting rid of excess inflammatory cells through natural cell death and phagocyte clearance. Frankincense, an oleogum resin of different species of the genus Boswellia (Burseraceae), has long been used in eastern countries' traditional medicine to alleviate pain and inflammation. Although it was demonstrated that boswellic acids are potent activators of polymorphonuclear cells, little is known about the effects of the total extract on the human phagocytes’ apoptosis. We here undertake a characterization of the Boswellia carteri resin extract (BCE) effects on human neutrophils activity and viability in vitro. Oxidative burst after stimulation with BCE was evaluated by reduction of nitroblue tetrazolium (NBT) colorimetric method for superoxide anion radical in the the presence of different compounds (N-ethyl maleimide, diltiazem, chelerythrine and wortmannin). Neutrophils viability was assessed by MTT. Flow analyses were performed with propidium iodide (PI) and annexin V-FITC. Our results show that BCE induces the release reactive species of oxygen in human neutrophils in a dose dependent manner. The superoxide anion radical is principally produced via NADPH oxidase since inhibitors of the enzyme may prevent it. Ca+2 depletion reduce the magnitude of activation and PKC and PI3-K are also apparently involved in the process. The BCE has also cytotoxic activity revealed by the MTT assay. This effect seems to be produced by an apoptotic process as shown by the exclusion of the dye propidium iodide and the annexin V-FITC binding. The capacity of the Boswellia carterii extract to accelerate the cellular death by an apoptotic process suggest that either the extract or its active compounds could have applications for the …
subject: Original Articles
  topic: section
creator: Martín Dadé
creator: Jose Prieto-Garcia
creator: Flavio Fancini
creator: Guillermo Raúl Schinella
rights: Copyright (c) 2023 Guillermo Raúl Schinella, José María Prieto, Flavio Fancini, Martín Dadé https://creativecommons.org/licenses/by/4.0
date: 2024-01-31
publication date: 2024-01-31
doi: 10.24377/jnpd.article2180

LEGALISING DRUGS OF ABUSE: THE LE CHATELIER THOUGHT EXPERIMENT
type: item
description: Background There is currently much debate on legalising some psychoactive materials such as Cannabis. This could stabilise policing costs, reduce crime, generate tax revenue and control sale by registration of outlets. Such outcomes are valuable, but legalisation may have unintended consequences that are deleterious to society or have no effects on crime or user’s health. Here, I propose that behaviour following changes to control of possession and supply may have outcomes that could be predicted by the equilibrium law proposed by Henry Le Chatelier. This law describes a system moving in such a way as to remove the constraints placed upon it. Aims The purpose of this opinion paper is to follow Le Chatelier’s Principle (LCP) as a thought experiment to predict the outcomes (products) of drug consumption (reactants) when factors influencing this consumption (conditions) are changed. If this equilibrium law can be applied to human behaviour, it may have value to predict the outcomes of these conditions and assess whether they are positive or injurious. It must be noted that the views expressed here are solely those of the author. Methods Pubmed, ScienceDirect, SpringerLink and Google were used for literature searches and “drugs” will be used for drugs of abuse and distinct from medicinal drugs, apart from methadone and heroin (diamorphine). Results Applying LCP predicts that drug use may not necessarily decrease, but availability, poor health and criminal activity may increase use. The businesses behind drug treatment and illegal distribution have too much financial incentive to halt operations. Conclusion Organisations recommending legislative changes to drug control should conduct thought experiments to horizon scan for unintended consequences, that may be costly and deleterious to society. Governments often focus too heavily on the evidence base as the sole driver for change. However, common sense drawn from opinion from these experiments may lead to wisdom. Keywords…
subject: Opinion papers
  topic: section
subject: War on drugs
  topic: keywords
subject: Le Chatelier’s Principle
  topic: keywords
subject: psychoactive substances
  topic: keywords
subject: regulation
  topic: keywords
subject: legalisation
  topic: keywords
subject: drugs of abuse
  topic: keywords
creator: Simon Gibbons
rights: Copyright (c) 2023 Simon Gibbons https://creativecommons.org/licenses/by/4.0
date: 2023-12-29
publication date: 2023-12-29
doi: 10.24377/jnpd.article1326

Quantification of Linalool in 3D Printed Fast-Dissolving Oral Films by A High-Pressure Liquid Chromatography Method
type: item
description: Introduction: Linalool has shown inhibitory effects against Candida albicans. Microbial resistance is developing towards the current antifungal drugs. Therefore, an oral formulation of linalool oil may be used to effectively treat oral thrush. A wide range of patients can use fast-dissolving oral films (FDFs). Three-dimensional printing (3DP) may be utilised for the manufacture of FDFs. Aims: to formulate linalool in FDFs and quantify it using high-pressure liquid chromatography (HPLC). Methods: A powder formulation containing linalool (5%w/w) was prepared and filaments were produced at 75°C and printed at 185°C. The films were dissolved either in methanol or deionised water, and linalool was quantified in the aqueous solutions. The mobile phase of a previously reported HPLC method was modified to quantify linalool in the aqueous solutions. The HPLC method was validated by measuring linalool in standard methanol and aqueous solutions. Results: Preparing aqueous solutions of filaments and films provided less variability in the analyses. 3D-printed FDFs had an average weight of 78.44 ± 6.84 mg. Applying the HPLC method revealed that the amounts of linalool changed from a theoretical 25 mg (per 0.5 g of filament) to the range of 23.98 ± 1.22 to 33.79 ± 2.43 mg. In contrast, the amounts of linalool were changed in films from theoretical 25 mg (per 0.5 g of film) to 13.82 ± 3.24 mg to 21.04 ± 0.92 mg. These observations indicated the evaporation of linalool considerably during printing at 185 °C. Conclusion: This work found that linalool FDFs should be printed at temperatures lower than 185 °C and dissolved in deionised water for better HPLC analytical consistency.
subject: Original Articles
  topic: section
subject: 3D printing; HPLC; Linalool; Lavender oil; Thrush; fast dissolving oral films
  topic: keywords
creator: Touraj Ehtezazi
creator: Asmaa Kteich
creator: Rana Abdulkarim
creator: Rachael Lynch
creator: Marwan Algellay
creator: Alice P McCloskey
creator: Matthew Roberts
creator: Satyajit D Sarker
rights: Copyright (c) 2023 Touraj Ehtezazi, Asmaa Kteich, Rana Abdulkarim, Rachael Lynch, Marwan Algellay, Alice P McCloskey, Matthew Roberts, Satyajit D Sarker https://creativecommons.org/licenses/by/4.0
date: 2023-10-20
publication date: 2023-10-20
doi: 10.24377/jnpd.article845

How specific is Crispr-Cas9?
type: item
subject: Letters to the Editor
  topic: section
subject: Crispr-Cas9
  topic: keywords
subject: genetic engineering
  topic: keywords
subject: calystegines
  topic: keywords
subject: plant secondary metabolism
  topic: keywords
creator: Robert J. Nash
rights: Copyright (c) 2023 Robert J. Nash https://creativecommons.org/licenses/by/4.0
date: 2023-10-20
publication date: 2023-10-20
doi: 10.24377/jnpd.article1622

The Proceedings of the 4th CNPD Conference
type: item
description: The 4th Annual CNPD (Centre for Natural Products Discovery) Conference was held on 19-22 June 2023 at the John Lennon Arts and Design Building (Liverpool John Moores University). The Organisation Committee has kindly approved the publication of all communications presented to this event in this issue of the Journal of Natural Products Discovery. This issue will replace the classic “Book of Abstracts” that was published in previous years. Therefore, authors will have a collective D.O.I. associated with their presentations instead of an ISSN. We hope that in this way both the event and splendid work of the attendants will be more publicly accessible. Please, follow this example to cite any of the abstracts: Gibbons, S. (2023). " Phytochemistry: not just for drugs and busting bugs." Journal of Natural Products Discovery 2(1): 2. Prof. Dr. Satyajit D. Sarker Editor in Chief Dr. Jose M. Prieto-Garcia Executive Editor Dr Alistair Fielding Member of the Editorial Committee
subject: Conference Abstracts
  topic: section
subject: Natural Products
  topic: keywords
subject: Food
  topic: keywords
subject: Medicines
  topic: keywords
subject: Cosmetics
  topic: keywords
creator: Conference Series Centre for Natural Products Discovery
rights: Copyright (c) 2023 Conference Series Centre for Natural Products Discovery https://creativecommons.org/licenses/by/4.0
date: 2023-10-17
publication date: 2023-10-17
doi: 10.24377/jnpd.article1911

THE 4th Annual CNPD (Centre for Natural Products Discovery) Conference
type: item
description: The 4th Annual CNPD (Centre for Natural Products Discovery) Conference held on 19-22 June 2023 at the John Lennon Arts and Design Building, has been a remarkable success. The feedback from the participants from all over the world (e.g., Bangladesh, Czech Republic, China, Pakistan, India, Italy, Nepal, Romania, South Korea, Switzerland, The Philippines, Turkey and USA), as well as from our home participants, was simply excellent. Many commented that our conference is one of the best conferences they have ever attended. Prof Laura Bishop, PVC Science opened the conference, and welcomed the participants on behalf of the Faculty and LJMU. Prof Satya Sarker, Director of School of Pharmacy and Biomolecular Sciences, and the Founding Head of the Centre for Natural Products Discovery, presented an overview of the centre and welcomed everybody on behalf of the School and the Centre. During the conference, several outstanding invited talks were presented and the quality of the short 10 min presentations was also excellent. The poster session was lively and highly interactive, the extended tea/coffee breaks and lunch breaks provided a fantastic opportunity for networking. There was a lively panel discussion on the contemporary issues in natural products research, and the impact of AI on natural products research and scientific publications dominated the discussion. There were three prizes sponsored by Goss Scientific, and Perkin Elmer, given to the best poster, the best short oral presentation and the best overall performance, selected by a panel of four judges, two internal and two externals. We are absolutely delighted to see our PhD student Waed Alsheikh won the best oral and overall best performance prizes. It can be mentioned that Waed is the Faculty Winner of the 3 min thesis presentation, and the LJMU winner through popular votes. The conference concluded with my ‘vote of thanks’, and a declaration that the 5th Annual CNPD conference will be held online over Teams in 20…
subject: Editorial
  topic: section
subject: Conference
  topic: keywords
subject: Natural Products
  topic: keywords
creator: Satyajit Sarker
rights: Copyright (c) 2023 Prof. Satya Sarker https://creativecommons.org/licenses/by/4.0
date: 2023-10-17
publication date: 2023-10-17
doi: 10.24377/jnpd.article1847

PHYTOCHEMICAL, ELEMENTAL ANALYSIS AND ANTIFUNGAL ACTIVITY OF ERIGERON FLORIBUNDUS (BILBAO FLEABANE) LEAF EXTRACT AGAINST MALASSEZIA FURFUR
type: item
description: Background: Elemental composition and phytochemical content of plants viz; saponins, flavonoids, alkaloids, tannins, polyphenols, terpenoids and their concentrations are potentials for their antifungal or antimicrobial activity. Aim: This study is aimed at evaluating the phytochemical, elemental composition and antifungal activity of the essential oil and dichloromethane extract of E. floribundus, against Malassezia furfur - the fungi that causes Pityriasis versicolor (eczema). Method: Assays for the antifungal activity of were performed using the agar dilution method at serial concentrations ranging from 2 to 0.06 mg/ml. Phytochemicals were determined using the suitable Official Analytical (AOAC) methods. Results: The essential oil of E. floribundus was the most potent, inhibiting the fungi at 0.25 mg/ml (IZD = 27 mm), while the dichloromethane extract inhibited the organism at 0.5 mg/ml (IZD = 25 mm), showing a better activity than the control antifungal, clotrimazole which showed an MIC of 1 mg/ml and an inhibition zone diameter (IZD) of 18 mm. Saponins showed the highest concentration of 900 mg per 5 g in the phytochemicals analyzed; with tannins showing the lowest of 56.6 mg per 5 g. The concentrations of carbon, nitrogen, magnesium, sodium and Sulphur were 68.7 mg per 100 g, 49.4 mg per 100 g, 324.5 ppm, 78.50 ppm and nil respectively. Conclusion: The essential oil and dichloromethane extract of E. floribundus revealed a good potential as an antifungal agent against M. furfur based on their minimum inhibition concentration, which is better than the conventional antifungal agent used as control in this study. Concentrations of phytochemicals in the plant also suggest that it has potentials for the traditional treatment of eczema/skin disorders.
subject: Original Articles
  topic: section
subject: Antifungal activity
  topic: keywords
subject: Erigeron floribundus
  topic: keywords
subject: Phytochemicals
  topic: keywords
subject: Malassezia furfur
  topic: keywords
creator: Michael Akomaye Akpe
creator: Alfred David Enang
creator: Faith Sutu Patrick-Inezi
creator: David Ibitham Edodi
rights: Copyright (c) 2024 Michael Akomaye Akpe, Alfred David Enang, Faith Sutu Patrick-Inezi, David Ibitham Edodi https://creativecommons.org/licenses/by/4.0
date: 2024-07-12
publication date: 2024-07-12
doi: 10.24377/jnpd.article871

APPLICATIONS OF ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPY IN NATURAL PRODUCT RESEARCH
type: item
description: Background Applications of electron paramagnetic resonance (EPR) spectroscopy to natural product research have proven useful to understand the properties of natural product extracts and their intrinsic compounds. There are few reviews on this subject written as an initial introduction. Aims To provide an explanatory review of applications of EPR to natural products research. Methods The search for this review was carried out in the following databases: SciFinder, Google Scholar, PubMed, MEDLINE, and Science Direct, with particular emphasis on publications from the last decade. Results The articles chosen showed qualitative and quantitative data about the applications of EPR in the natural product research area. Studies include the use to measure radical scavenging capacity and direct detection of radicals in extracts. Other applications include the study of autooxidation, and photochemical properties. Conclusion This review brings together many examples highlighting methods by which EPR spectroscopy is useful in natural products research as a guide for future use.
subject: Review Articles
  topic: section
subject: EPR
  topic: keywords
subject: natural products
  topic: keywords
subject: extracts
  topic: keywords
subject: quinones
  topic: keywords
creator: Alistair John Fielding
rights: Copyright (c) 2022 Alistair John Fielding
date: 2022-12-23
publication date: 2022-12-23
starting page: 16
ending page: 16
doi: 10.24377/jnpd.article725

https://azljmu07a0101.ljmu-eprints.cdl.cosector.com/JNPD/article/view/732
type: item
title: DICHLOROMETHANE EXTRACT OF THE LEAVES OF ARBUTUS PAVARII PAMP. EXHIBITS CYTOTOXICITY AGAINST THE PROSTATE CANCER CELL LINE PC3: A BIOASSAY-GUIDED ISOLATION AND IDENTIFICATION OF ARBUTIN AND BETULINIC ACID METHYL ESTER
description: Background: Arbutus pavarii Pamp. (fam. Ericaceae), commonly known as “Shmeri”, “Shmar” and “Libyan Strawberry”, is an endemic Libyan medicinal plant, growing almost exclusively in the Al-Jabel Al-Akhdar mountainous region in Libya. Aims: To assess the cytotoxicity of A. pavarii against human cancer cell lines and to carry out bioassay-guided isolation and identification of compounds. Materials and Methods: Shed-dried and ground leaves of A. pavarii were Soxhlet-extracted, successively, with n-hexane, dichloromethane (DCM) and methanol (MeOH), and assessed for cytotoxicity against several human cancer cell lines using the MTT assay. The cytotoxicity of the DCM extract against the normal human prostate cell line PNT2 was also assessed to determine the selectivity index (SI). The DCM extract was subjected to vacuum liquid chromatography (VLC) to produce eight fractions. A reversed-phase preparative HPLC analysis of the active VLC fraction was carried out to purify the major compounds present in the active fraction, and the structures of the compounds were elucidated by spectroscopic means. Results: The DCM extract was more cytotoxic against the PC3 cell line (IC50= 26 mg/mL) but less to the normal human prostate cell line PNT2 (IC50 = 90 mg/mL) with a selectivity index of 3.5. VLC analysis produced 8 fractions, with fraction VLC-5 most active against PC3 cells. Prep-HPLC-based purification of VLC-5 afforded the isolation of arbutin (1) and betulinic acid methyl ester (2), the structures of which were elucidated by spectroscopic means. Conclusion: The DCM extract of the leaves of A. pavarii exhibited significant cytotoxicity to PC3 cells, but much less cytotoxicity against normal human prostate cell line. The isolated compounds from the active fraction, arbutin (1) and betulinic acid methyl ester (2), which were previously shown to possess cytotoxic properties, could be responsible for the cytotoxicity of the DCM extract.
subject: Original Articles
  topic: section
subject: Arbutus pavarii
  topic: keywords
subject: Ericaceae
  topic: keywords
subject: Cytotoxicity
  topic: keywords
subject: Cancer
  topic: keywords
subject: Arbutin
  topic: keywords
subject: Betulinic acid
  topic: keywords
subject: Cell line
  topic: keywords
creator: Satyajit D. Sarker
creator: Afaf Al Groshi
creator: Lutfun Nahar
creator: Fyaz M. D. Ismail
creator: Andrew R. Evans
rights: Copyright (c) 2022 Satyajit D. Sarker, Afaf Al Groshi, Lutfun Nahar, Fyaz M. D. Ismail, Andrew R. Evans
date: 2022-12-30
publication date: 2022-12-30
starting page: 9
ending page: 9
doi: 10.24377/jnpd.article732

EFFECTS OF IMPORTANT PLANT SPECIES OF TERUEL ON THE PRO-INFLAMMATORY SIGNALLING CASCADE OF HUMAN MONOCYTES.
type: item
description: Background: The study of medicinal and edible plants from Teruel (Spain) –one of the biodiverse regions of the Mediterranean- has been particularly limited to the writing up and analysis of taxonomical databases and lists of protected species. The following analysis and work will be the first of its nature test their anti-inflammatory activity can be successfully based on information gained through knowledge on their traditional use.
subject: Original Articles
  topic: section
subject: Anti-inflammatory Antioxidant Phenols Ethnopharmacology
  topic: keywords
creator: Michelle Rocio Viteri
rights: Copyright (c) 2022 Michelle Rocio Viteri
date: 2022-12-30
publication date: 2022-12-30
starting page: 12
ending page: 12
doi: 10.24377/jnpd.article700

OLAX SUBSCORPIOIDEA OLIV. (OLACACEAE): AN ETHNOMEDICINAL AND PHARMACOLOGICAL REVIEW
type: item
description: Background Olax subscorpioidea Oliv. (Olacaceae) is a woody shrub that is widely distributed in Africa. It has trado-medicinal importance and is used in the treatment of asthma, cancer, convulsion, diabetes, intestinal worm infections, jaundice, mental illnesses, neurodegenerative disorders, sexually transmitted infections, swellings and rheumatism, and yellow fever. Aims To review available literature on the phytochemistry, ethnobotany, pharmacology and toxicity of Olax subscorpioidea Oliv. Methods Published findings were searched in online databases such as Web of Science, Scopus, Pubmed, Google Scholar and other relevant sources, and the data were sorted by relevance. Combinations of keywords used in the search include Olax subscorpioidea, Olacaceae, Olax, Ewe Ifon, and African medicinal plants. Results The presence of alkaloids, anthraquinones, cardiac glycosides, flavonoids, phenolic compounds, proanthocyanidins, saponins, tannins and triterpenes has been reported from O. subscorpioidea. Cytotoxic santalbic acid was isolated from the methanol extract of the seed of this plant, while GC-MS and HPLC analyses of the n-butanol and n-hexane extracts of the leaf revealed the presence of caffeic acid, quercetin, morin, rutin, n-hexadecanoic acid (palmitic acid), 7,10,13-hexadecatrienoic acid and methyl ester, hentriacontane, 9,17-octadecadienal (Z)-, 9,12-octadecadienoic acid (Z,Z)-, squalene, nonacosane, octadecanoic acid. Bioactivity studies on this plant demonstrated its medicinal potential mainly as an analgesic, anthelmintic, anti-arthritic, antidepressant, antihyperglycaemic, anti-inflammatory, antioxidant, antimalarial and antimicrobial agent. Oral acute toxicity of the leaf extracts in rats appeared to be greater than 5,000 mg/kg body weight. Conclusion Published literature available to date on O. subscorpioidea provides some preliminary scientific basis for the ethnomedicinal uses of this plant. However, some ethnomedicinal uses have not been scientifically v…
subject: Review Articles
  topic: section
subject: Olax subscorpioidea, Olacaceae, anticancer, Anti-nociceptive, Phytochemicals, medicinal plants
  topic: keywords
creator: Yemi Adekola Adekunle
creator: Babatunde B Samuel
creator: Amos A Fatokun
creator: Lutfun Nahar
creator: Satyajit D Sarker
rights: Copyright (c) 2022 Yemi Adekola Adekunle, Babatunde B Samuel, Amos A Fatokun, Lutfun Nahar, Satyajit D Sarker
date: 2022-07-25
publication date: 2022-07-25
starting page: 15
ending page: 15
doi: 10.24377/jnpd.article673

NUTRITIVE AND MEDICINAL VALUE OF GONGRONEMA LATIFOLIUM
type: item
description: Background The tropical rainforest plant Gongronema latifolium is popular for its nutritive and medicinal value across many African nations. The rich phytochemistry of this plant is responsible for a wide variety of pharmacological actions. It is commonly used as a vegetable in soups, salads or as a food spice in other food preparations. Medicinally, it is widely used in diabetes, malaria, hepatitis, stomachache, anorexia, cough etc. Aims This essay aims to cover details about the origin, botanical features, ethnopharmacological uses, indigenous rights, phytochemical profile and pharmacological properties of Gongronema latifolium. Methods PubMed and Google Scholar databases were searched for the name “Gongronema Latifolium”. Results & Conclusion This short review tried to justify the ethnomedical importance of G.latifolium for researchers. It is enriched with varities of flavonoids, saponins,alkaloids and steroidal phytochemicals which exhibit prominent pharmacological actions such as hypoglycaemic, hypolipidemic, cytotoxic, anti-oxidant and antimicrobial in-vitro and in-vivo. One of the active compound; iloneoside was able to show potent antileukemic activity. Itshould be evaluated against other cancer cell lines. Lastly, further research is required to understand the true potential of this African plant.
subject: Mini-Reviews
  topic: section
subject: Gongronema latifolium, Ethnopharmacological, Phytochemistry, Ileonoside, Anti-cancer
  topic: keywords
creator: Moinuddin Abdul Khalid Amrelia
rights: Copyright (c) 2022 Moinuddin Abdul Khalid Amrelia
date: 2022-12-12
publication date: 2022-12-12
starting page: 14
ending page: 14
doi: 10.24377/jnpd.article751